Laurence Florens

Career Trajectory

Research Cluster Map

Direct collaborators. Node size = influence, colour = community, dashed = past collaboration.

• Multicomplex Integrative Structural Modeling of a Human Histone Deacetylase Interactome

  bioRxiv (Cold Spring Harbor Laboratory) · 2026-03-17

• The human parasite, <i>Toxoplasma gondii,</i> is paralyzed without two components of the apical polar ring

  bioRxiv (Cold Spring Harbor Laboratory) · 2026-03-09

• The Spatiotemporal Proteome Landscape of Aging: Structural determinants of age-sensitive proteome remodeling

  bioRxiv (Cold Spring Harbor Laboratory) · 2026-03-01

• Proteomic analysis of FACS-enriched whole nematocysts from the colonial hydrozoan Hydractinia symbiolongicarpus

  Toxicon X · 2026-02-04

• Histone fold domain positioning dictates cotranslational heterodimeric assembly of paralogous TAF12/TAF12L in Candida albicans

  Journal of Biological Chemistry · 2026-02-04

• p62 protein pleomorphism confers tissue-specific function

  bioRxiv (Cold Spring Harbor Laboratory) · 2026-01-11

• MassIVE MSV000100481 - The Proteome Landscape of Replicative Aging: Spatiotemporal Dynamics Across Cellular Compartments

  2026-01-01

• MassIVE MSV000100482 - The Proteome Landscape of Replicative Aging: Spatiotemporal Dynamics Across Cellular Compartments

  2026-01-01

• MassIVE MSV000100718 - Crosslinking mass spectrometry analysis of C-terminally Halo-tagged HDAC1 and HDAC2 stably expressed in Flp-In-293 cells

  2026-01-01

• MassIVE MSV000100477 - The Proteome Landscape of Replicative Aging: Spatiotemporal Dynamics Across Cellular Compartments

  2026-01-01

• MassIVE MSV000100456 - The Proteome Landscape of Replicative Aging: Spatiotemporal Dynamics Across Cellular Compartments

  2026-01-01

• MassIVE MSV000100479 - The Proteome Landscape of Replicative Aging: Spatiotemporal Dynamics Across Cellular Compartments

  2026-01-01

• MassIVE MSV000100457 - The Proteome Landscape of Replicative Aging Spatiotemporal Dynamics Across Cellular Compartments

  2026-01-01

• MassIVE MSV000100475 - The Proteome Landscape of Replicative Aging: Spatiotemporal Dynamics Across Cellular Compartments

  2026-01-01

• MassIVE MSV000100454 - The Proteome Landscape of Replicative Aging: Spatiotemporal Dynamics Across Cellular Compartments

  2026-01-01

• MassIVE MSV000100775 - Quantitative DIA proteomics analysis of Schmidtea mediterranea ECM samples from regenerating animals

  2026-01-01

• MassIVE MSV000100476 - The Proteome Landscape of Replicative Aging: Spatiotemporal Dynamics Across Cellular Compartments

  2026-01-01

• Affinity Purification Mass Spectrometry of HDAC1-Halo and HDAC2-Halo. For each replicate XLMS analysis, 3 confluent 850 cm2 culture vessels of Flp-In-293 cells stably expressing HDAC1-Halo or HDAC2-Halo were harvested, washed twice with ice-cold PBS, incubated at -80C until frozen, thawed, and resuspended in ice-cold lysis buffer containing: 20 mM HEPES (pH 7.5), 1.5 mM MgCl2, 0.42 M NaCl, 10 mM KCl, 0.2% Triton X-100, 0.5 mM DTT, 0.1 mM benzamidine HCL, 55 uM phenanthroline, 10 uM bestatin, 20 uM leupeptin, 5 uM pepstatin A, 1mM PMSF, and 500 units SAN. Following Dounce homogenization, extracts were incubated for 2 hours at 4C and centrifuged 40,000 x g for 30 minutes at 4C. The salt concentration was adjusted to 0.3 M NaCl by adding ice-cold buffer containing: 10 mM HEPES (pH 7.5), 1.5 mM MgCl2, 10 mM KCl, 0.5 mM DTT, 0.1 mM benzamidine HCl, 55 uM phenanthroline, 10 uM bestatin, 20 uM leupeptin, 5 uM pepstatin A, and 1mM PMSF. Lysates were centrifuged at 40,000 x g for 30 minutes at 4C for further analysis. Protein extracts were incubated with 200 ul Magne HaloTag bead slurry and crosslinked with disuccinimidyl sulfoxide (DSSO, Cayman Chemical Company) MS-cleavable crosslinker. DSSO was added to samples while the proteins were immobilized on beads to a final concentration of 5 mM. Samples were incubated at room temperature for 40 min. Reactions were quenched with the addition of NH4CO3 to a final concentration of 50 mM and samples were incubated an additional 15 min. Bound proteins were eluted by on-beads digestion with AcTEV Protease (Invitrogen) overnight at 25C. Crosslinking Mass Spectrometry and Data Analysis. Digested peptides were analyzed with an Orbitrap Fusion Lumos (Thermo Fisher) coupled to a Dionex U3000 HPLC using previously described data acquisition settings. For each .RAW file, the XlinkX node in Proteome Discoverer v2.4 was used to identify MS2 fragmentation scans with reporter ions characteristic of DSSO crosslinked peptides. Peak lists were searched against a human proteome database (Genome Reference Consortium Human Build 38 patch release 13) containing 44519 unique protein sequences, 426 of which were contaminant proteins. The database was searched for fully tryptic peptides, allowing for a maximum of 2 missed cleavages and a minimum peptide length of 5 amino acids. Precursor mass tolerance, FTMS fragment mass tolerance, and ITMS Fragment tolerance, were set to 10 ppm, 20 ppm, and 0.5 Da, respectively. Xlink Validator FDR threshold was set to 0.01.

  Open MIND · 2026-01-01

• MassIVE MSV000100412 - The Proteome Landscape of Replicative Aging: Spatiotemporal Dynamics Across Cellular Compartments

  2026-01-01

• MassIVE MSV000100480 - The Proteome Landscape of Replicative Aging: Spatiotemporal Dynamics Across Cellular Compartments

  2026-01-01